Spec. IgE S (100 Tests)

100 Tests

Ref.: 36070000

Enzyme immunoassay for the quantitative determination of allergen-specific IgE in human serum or plasma.

The quantitative determination of circulating spec. IgE in serum/plasma is performed by a non-competitive enzyme immunoassay. The solid phase consists of a chemically activated paper disc to which the corresponding allergens are covalently bound. In the first step, patient serum or plasma is pipetted onto the allergen disc. This results in binding of the allergen-specific IgE to the solid-phase-bound allergen. Excess serum/plasma is then removed in a washing step. In the second step, an enzyme-labeled anti-human IgE is added to the allergen disc containing the allergen-IgE complex. This results in binding of the labeled anti-human IgE to the solid phase bound specific IgE. Unbound anti-human IgE is removed in a wash step. The amount of bound, labeled anti-human IgE is proportional to the amount of specific IgE in the serum/plasma. In the next step, a substrate solution (p-nitrophenyl phosphate) is added. The activity of alkaline phosphatase results in a colored solution. The enzyme reaction is stopped at the end of the incubation period with a stop solution. The absorbances of the colored solutions are measured in the photometer. The evaluation is performed using a calibration curve consisting of the absorbance values of the measured calibrator cavities.

• > 500 allergens available
• Of which 25 allergen components for molecular allergy diagnostics

Component-based allergy diagnostics

• Detection of primary sensitization
• Identification of risk markers
• Differentiation of a true
• sensitization versus a cross-reaction